Multiplex GPCR Assay in Reverse Transfection Cell Microarrays
نویسندگان
چکیده
منابع مشابه
Quantitative analysis of highly parallel transfection in cell microarrays.
As more genomes are sequenced, we are facing the challenge of rapidly unraveling the functions of genes. To that end, cell microarrays have recently been described that transfect thousands of nucleic acids in parallel and can be used to analyze the phenotypic consequences of such perturbations. As many parameters can influence the efficacy of transfection in such a format, we describe some impo...
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We established a subclone, SHOK, from the GHE-L cell line, an immortal line derived from a primary culture of Syrian hamster embryo cells, as a recipient cell line useful for the detection of oncogenes by transfection. SHOK cells were almost as susceptible as NIH 3T3 cells to focus formation by many oncogenes, including v-raf, v-Ha-ras, v-Ki-ras, or activated c-Ha-ras. The susceptibility of SHO...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2004
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057103261880